XLD (Xylose Lysine Deoxycholate) agar (P/N: 1052870500)

XLD (Xylose Lysine Deoxycholate) agar (P/N: 1052870500)

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Specified by ISO 6579, ISO FDIS 6579,     ISO 19250, APHAGranuCult™ XLD agar acc. ISO 6579
Yeast Extract3 g/lYeast Extract3 g/l
NaCl5 g/lNaCl5 g/l
Xylose3.75 g/lD(+)-Xylose3.75 g/l
Lactose7.5 g/lLactose7.5 g/l
Sucrose7.5 g/lSucrose7.5 g/l
L-Lysine Hydrochloride5 g/lL(+)-Lysine5 g/l
Sodium Thiosulfate6.8 g/lSodium Thiosulfate6.8 g/l
Iron(III) Ammonium Citrate0.8 g/lAmmonium Iron(III) Citrate0.8 g/l
Phenol Red0.08 g/lPhenol Red0.08 g/l
Sodium Deoxycholate1 g/lSodium Deoxycholate1 g/l
Agar9-18 g/lAgar-Agar*14.5 g/l
Water1000 ml/lWatern/a
pH at 25 °C7.4 ± 0.2pH at 25 °C7.4 ± 0.2
* Agar-Agar is equivalent to other different terms of agar.

Dissolve  55  g  in  1  l  of  purified  water.  Heat  in  boiling  water  and  agitate  frequently until  completely  dissolved. Do not autoclave! Precipitates may be formed but do not affect the performance of the culture medium.
Preparation  of  large  volumes,  overheating  and  prolonged  storage  in  water  bath  (47-50
°C) is to be avoided.
The prepared medium is clear and red. The pH value at 25 °C is in the range of 7.2-7.6.
There should be no visible moisture on the plates before use. When moisture is present, the  plates  should  be  dried  for  the  minimum  time  required  to  remove  visible  moisture, following the procedure as described by EN ISO 11133.