
EC (Escherichia coli) Broth (P/N: 1107650500)
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Email: Info@chanu.com.vn
Mô tả
* Enzymatic digest of casein is equivalent to trypticase.
** Bile salts mixture includes bile salts No. 3 (see EN ISO 11133).
Specified by ISO 7251 | Specified by FDA-BAM M49 | GranuCultTM EC (Escherichia coli) Broth acc. ISO 7251 and FDA-BAM | |||||||||||
Enzymatic Digest of Casein | 20 g/l | Trypticase or Tryptose | 20 g/l | Enzymatic Digest of Casein* | 20 g/l | ||||||||
Lactose | 5 g/l | Lactose | 5 g/l | Lactose | 5 g/l | ||||||||
Bile Salts No. 3 | 1.5 g/l | Bile Salts No. 3 | 1.5 g/l | Bile Salts Mixture** | 1.5 g/l | ||||||||
K2HPO4 | 4 g/l | K2HPO4 | 4 g/l | K2HPO4 | 4 g/l | ||||||||
KH2PO4 | 1.5 g/l | KH2PO4 | 1.5 g/l | KH2PO4 | 1.5 g/l | ||||||||
NaCl | 5 g/l | NaCl | 5 g/l | NaCl | 5 g/l | ||||||||
Water | 1000 ml/l | Water | 1000 ml/l | Water | n/a | ||||||||
pH at 25 °C | 6.8 ± 0.2 | pH at 25 °C | 6.9 ± 0.2 | pH at 25 °C | 6.9 ± 0.2 |
** Bile salts mixture includes bile salts No. 3 (see EN ISO 11133).
Dissolve 37 g in 1 l of purified water. Dispense into tubes containing Durham tubes. Autoclave 15 min at 121 °C. The Durham tubes shall not contain any air bubbles after autoclaving.
The prepared medium is clear and yellowish-brown.