XLD (Xylose Lysine Deoxycholate) agar (P/N: 1052870500)
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Mô tả
* Agar-Agar is equivalent to other different terms of agar.
Specified by ISO 6579, ISO FDIS 6579, ISO 19250, APHA | GranuCult™ XLD agar acc. ISO 6579 | |||||||||||||||
Yeast Extract | 3 g/l | Yeast Extract | 3 g/l | |||||||||||||
NaCl | 5 g/l | NaCl | 5 g/l | |||||||||||||
Xylose | 3.75 g/l | D(+)-Xylose | 3.75 g/l | |||||||||||||
Lactose | 7.5 g/l | Lactose | 7.5 g/l | |||||||||||||
Sucrose | 7.5 g/l | Sucrose | 7.5 g/l | |||||||||||||
L-Lysine Hydrochloride | 5 g/l | L(+)-Lysine | 5 g/l | |||||||||||||
Sodium Thiosulfate | 6.8 g/l | Sodium Thiosulfate | 6.8 g/l | |||||||||||||
Iron(III) Ammonium Citrate | 0.8 g/l | Ammonium Iron(III) Citrate | 0.8 g/l | |||||||||||||
Phenol Red | 0.08 g/l | Phenol Red | 0.08 g/l | |||||||||||||
Sodium Deoxycholate | 1 g/l | Sodium Deoxycholate | 1 g/l | |||||||||||||
Agar | 9-18 g/l | Agar-Agar* | 14.5 g/l | |||||||||||||
Water | 1000 ml/l | Water | n/a | |||||||||||||
pH at 25 °C | 7.4 ± 0.2 | pH at 25 °C | 7.4 ± 0.2 |
Dissolve 55 g in 1 l of purified water. Heat in boiling water and agitate frequently until completely dissolved. Do not autoclave! Precipitates may be formed but do not affect the performance of the culture medium.
Preparation of large volumes, overheating and prolonged storage in water bath (47-50
°C) is to be avoided.
The prepared medium is clear and red. The pH value at 25 °C is in the range of 7.2-7.6.
There should be no visible moisture on the plates before use. When moisture is present, the plates should be dried for the minimum time required to remove visible moisture, following the procedure as described by EN ISO 11133.