Plate Count Agar (PCA) (P/N: 1054630500)
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Hàng mới 100%
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Liên hệ: 0918 376 013 (Mr. Phương)
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Email: Info@chanu.com.vn
| Specified by ISO 4833, ISO 17410 | Specified by BAM M124, APHA | GranuCult™ Plate Count Agar acc. ISO 4833, ISO 17410 and FDA-BAM | ||||||||||||
| Enzymatic Digest of Casein | 5 g/l | Tryptone | 5 g/l | Enzymatic Digest of Casein** | 5 g/l | |||||||||
| Yeast Extract | 2.5 g/l | Yeast Extract | 2.5 g/l | Yeast Extract | 2.5 g/l | |||||||||
| Glucose* | 1 g/l | Dextrose | 1 g/l | D(+)-Glucose | 1 g/l | |||||||||
| Agar | 9-18 g/l | Agar | 15 g/l | Agar-Agar*** | 14 g/l | |||||||||
| Water | 1000 ml/l | Water | 1000 ml | Water | n/a | |||||||||
| pH at 25 °C | 7.0 ± 0.2 | pH at 25 °C | 7.0 ± 0.2 | pH at 25 °C | 7.0 ± 0.2 | |||||||||
Dissolve 22.5 g in 1 l of purified water. Heat in boiling water and agitate frequently until completely dissolved. Autoclave 15 min at 121 °C.
If the medium is to be used immediately for poured plate technique, cool it to 44-47 °C in a water bath before use. Use the molten medium as soon as possible, it should not be retained for more than 4 h, as specified by EN ISO 4833 and EN ISO 11133.
If the medium is used for surface plating technique, there should be no visible moisture on the plates before use. When moisture is present, the plates should be dried for the minimum time required to remove visible moisture, following the procedure as described by EN ISO 11133.
The prepared medium is clear and yellowish. The pH value at 25 °C is in the range of 6.8-7.2.
When dairy products are examined, add skim milk powder at 1 g/l of the culture medium prior to sterilization. The skimmed milk powder shall be free from inhibitory substances. GranuCult™ Plate Count Skimmed Milk Agar (article number 115338) already contains skim milk powder free from inhibitors.
