Plate Count Agar (PCA) (P/N: 1054630500)

Plate Count Agar (PCA) (P/N: 1054630500)

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Mô tả
Specified by ISO 4833, ISO 17410Specified by BAM M124, APHAGranuCult™ Plate Count Agar acc. ISO 4833, ISO 17410 and FDA-BAM
Enzymatic Digest of Casein5 g/lTryptone5 g/lEnzymatic Digest of Casein**5 g/l
Yeast Extract2.5 g/lYeast Extract2.5 g/lYeast Extract2.5 g/l
Glucose*1 g/lDextrose1 g/lD(+)-Glucose1 g/l
Agar9-18 g/lAgar15 g/lAgar-Agar***14 g/l
Water1000 ml/lWater1000 mlWatern/a
pH at 25 °C7.0 ± 0.2pH at 25 °C7.0 ± 0.2pH at 25 °C7.0 ± 0.2

Dissolve  22.5  g  in  1  l  of  purified  water.  Heat  in  boiling  water  and  agitate  frequently until completely dissolved. Autoclave 15 min at 121 °C.
If the medium is to be used immediately for poured plate technique, cool it to 44-47 °C in a  water  bath  before use. Use the molten medium as soon as possible, it should not be retained for more than 4 h, as specified by EN ISO 4833 and EN ISO 11133.
If the medium is used for surface plating technique, there should be no visible moisture on the plates before  use.  When  moisture  is  present,  the  plates  should  be  dried  for  the minimum  time  required  to  remove visible moisture, following the procedure as described by EN ISO 11133.
The prepared  medium  is  clear  and  yellowish.  The pH  value  at 25 °C  is  in the range of 6.8-7.2.
When  dairy  products  are  examined,  add  skim  milk  powder  at  1  g/l  of  the  culture medium  prior  to  sterilization.  The  skimmed  milk  powder  shall  be  free  from  inhibitory substances.  GranuCult™  Plate  Count   Skimmed   Milk   Agar   (article   number   115338) already  contains  skim  milk  powder  free  from  inhibitors.